Dr Sinead Miggin

Biology

Lecturer
MSc in Immunology & Global Health Coordinator

Biosciences
B3.14
(01) 7083855

Biography

Dr Sinead Miggin graduated in Toxicology from Athlone Institute of Technology. In 2000, she was awarded a PhD in Biochemistry which was undertaken at the Conway Institute, University College Dublin. She subsequently undertook a Postdoctoral Fellowship in Inflammation research at the Conway Institute (2000-2002) followed by a Senior Postdoctoral Fellowship at the Department of Biochemistry and Immunology, Trinity College Dublin, Ireland (2002-2006). She was appointed Lecturer in Immunology at the National University of Ireland Maynooth in 2006. In 2015, Dr Miggin was awarded a Postgraduate Diploma in Higher Education.

Research Interests

My research focuses on the molecular mechanisms involved in regulating the innate immune response through the Toll-like receptors (TLRs). TLRs play a critical role in the evolutionary conserved innate immune response and represents our first line of defense against invading pathogens. TLRs are activated in response to a broad spectrum of pathogen-associated molecular patterns (PAMPs) ranging from bacterial and viral components to fungal and protozoal molecules. Chronic Inflammatory Diseases such as Rheumatoid Arthritis (RA), Osteoarthritis (OA) and Type 2 Diabetes, whilst having apparently unrelated aetiologies, share a common late stage pathogenic process, characterized by increases in pro-inflammatory cytokines, such as TNFa, IL-1b, IL-6 and IL-18. During chronic inflammatory episodes, the 'in-born' or innate immune system may become dysregulated through mechanisms involving Toll-like receptors (TLRs). To better understand the inflammatory response, my group employ cutting edge proteomics technologies to identify novel modulators of TLR signalling and inflammatory processes. Thereafter, molecular immunological approaches are adopted to explore the role of newly identified proteins in the context of inflammatory conditions and antiviral immunity. In particular, my group are interested in the pathogenesis of Osteoarthritis and Type II Diabetes. My group are also interested in exploring how Oncoviruses, such as EBV, subvert the innate immune system. My group focus on the identification and functional characterisation of proteins that modulate inflammatory and antiviral immunity at the molecular level.

Peer Reviewed Journals

  Year Publication
2017 'Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications'
Gupta S;Maratha A;Siednienko J;Natarajan A;Gajanayake T;Hoashi S;Miggin S; (2017) 'Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications'. Scientific Reports, 7 (1) [DOI] [Details]
2017 'Long non-coding RNA AGER-1 functionally upregulates the innate immunity gene AGER and approximates its anti-tumor effect in lung cancer'
Pan Z;Liu L;Nie W;Miggin S;Qiu F;Cao Y;Chen J;Yang B;Zhou Y;Lu J;Yang L; (2017) 'Long non-coding RNA AGER-1 functionally upregulates the innate immunity gene AGER and approximates its anti-tumor effect in lung cancer'. Molecular Carcinogenesis, [DOI] [Details]
2015 'Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammatory and angiogenic mechanisms'
Connolly M.;Rooney P.;McGarry T.;Maratha A.;McCormick J.;Miggin S.;Veale D.;Fearon U. (2015) 'Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammatory and angiogenic mechanisms'. ANNALS OF THE RHEUMATIC DISEASES, [DOI] [Details]
2014 'The TIR-Domain Containing Adaptor TRAM Is Required for TLR7 Mediated RANTES Production'
Shevlin, E;Miggin, SM (2014) 'The TIR-Domain Containing Adaptor TRAM Is Required for TLR7 Mediated RANTES Production'. PLoS ONE, 9 [DOI] [Details]
2013 'TRIF-Mediated TLR3 and TLR4 Signaling Is Negatively Regulated by ADAM15'
Ahmed, S;Maratha, A;Butt, AQ;Shevlin, E;Miggin, SM (2013) 'TRIF-Mediated TLR3 and TLR4 Signaling Is Negatively Regulated by ADAM15'. Journal of Immunology, 190 :2217-2228 [DOI] [Details]
2011 'Targeted Disruption of Nonribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus'
O'Hanlon, KA;Cairns, T;Stack, D;Schrettl, M;Bignell, EM;Kavanagh, K;Miggin, SM;O'Keeffe, G;Larsen, TO;Doyle, S (2011) 'Targeted Disruption of Nonribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus'. Infection and Immunity, 79 :3978-3992 [DOI] [IR Link] [Details]
2011 'Absence of MyD88 Results in Enhanced TLR3-Dependent Phosphorylation of IRF3 and Increased IFN-beta and RANTES Production'
Siednienko, J;Gajanayake, T;Fitzgerald, KA;Moynagh, P;Miggin, SM (2011) 'Absence of MyD88 Results in Enhanced TLR3-Dependent Phosphorylation of IRF3 and Increased IFN-beta and RANTES Production'. Journal of Immunology, 186 :2514-2522 [DOI] [Details]
2011 'Nuclear Factor kappa B Subunits RelB and cRel Negatively Regulate Toll-like Receptor 3-mediated beta-Interferon Production via Induction of Transcriptional Repressor Protein YY1'
Siednienko, J;Maratha, A;Yang, S;Mitkiewicz, M;Miggin, SM;Moynagh, PN (2011) 'Nuclear Factor kappa B Subunits RelB and cRel Negatively Regulate Toll-like Receptor 3-mediated beta-Interferon Production via Induction of Transcriptional Repressor Protein YY1'. Journal of Biological Chemistry, 286 :44750-44763 [DOI] [Details]
2010 'TLR3-mediated IFN-beta gene induction is negatively regulated by the TLR adaptor MyD88 adaptor-like'
Siednienko, J;Halle, A;Nagpal, K;Golenbock, DT;Miggin, SM (2010) 'TLR3-mediated IFN-beta gene induction is negatively regulated by the TLR adaptor MyD88 adaptor-like'. European Journal of Immunology, 40 :3150-3160 [DOI] [Details]
2007 'NF-kappa B activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1'
Miggin, SM;Palsson-McDermott, E;Dunne, A;Jefferies, C;Pinteaux, E;Banahan, K;Murphy, C;Moynagh, P;Yamamoto, M;Akira, S;Rothwelll, N;Golenbock, D;Fitzgerald, KA;O'Neill, LAJ (2007) 'NF-kappa B activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1'. Proceedings of the National Academy of Sciences of the United States of America, 104 :3372-3377 [DOI] [IR Link] [Details]
2006 'New insights into the regulation of TLR signaling'
Miggin SM, O'Neill LA (2006) 'New insights into the regulation of TLR signaling'. Journal Of Leukocyte Biology, 80 :220-226 [Details]
2003 'Interferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappa'
Wietek, C., Miggin, S. M., Jefferies, C. A. and O’Neill, L.A. (2003) 'Interferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappa'. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 :50923-50931 [Details]
2002 'Characterization of the 5' untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the TP isoforms'
Coyle, AT., Miggin, SM. and Kinsella BT. (2002) 'Characterization of the 5' untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the TP isoforms'. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 :4058-4073 [Details]
2002 'Regulation of the extracellular signal regulated protein kinase cascades by the a and b isoforms of the human thromboxane A2 receptor'
Miggin, S. M. and Kinsella, B. T. (2002) 'Regulation of the extracellular signal regulated protein kinase cascades by the a and b isoforms of the human thromboxane A2 receptor'. MOLECULAR PHARMACOLOGY, 61 :817-831 [Details]
2002 'Investigation of a functional requirement for isoprenylation by the human prostacyclin receptor'
Miggin, S. M., Lawler, O. A. and Kinsella, B. T. (2002) 'Investigation of a functional requirement for isoprenylation by the human prostacyclin receptor'. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 :1714-1725 [Details]
2002 'Investigation of the mechanisms of G protein : effector coupling by the human and mouse prostacyclin receptors;  identification of critical species-dependent differences'
Miggin, S. M. and Kinsella, B. T. (2002) 'Investigation of the mechanisms of G protein : effector coupling by the human and mouse prostacyclin receptors;  identification of critical species-dependent differences'. JOURNAL OF BIOLOGICAL CHEMISTRY, 277 :27053-27064 [Details]
2001 'Thromboxane A(2) receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells'
Miggin, S.M. and Kinsella, BT. (2001) 'Thromboxane A(2) receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells'. BIOCHIMICA ET BIOPHYSICA ACTA, 1539 :147-162 [Details]
2001 'Protein kinase A dependent phosphorylation of serine 357 of the mouse prostacyclin receptor regulated its coupling to Gs-, Gi- and to Gq- coupled effector signalling'
Lawler, O. A., Miggin, S. M. and Kinsella, B. T. (2001) 'Protein kinase A dependent phosphorylation of serine 357 of the mouse prostacyclin receptor regulated its coupling to Gs-, Gi- and to Gq- coupled effector signalling'. JOURNAL OF BIOLOGICAL CHEMISTRY, 276 :33596-33607 [Details]
2001 'The effects of the statins lovastatin and cerivastatin on signalling by the prostanoid IP-receptor'
Lawler, O. A., Miggin, S. M. and Kinsella, B. T. (2001) 'The effects of the statins lovastatin and cerivastatin on signalling by the prostanoid IP-receptor'. BRITISH JOURNAL OF PHARMACOLOGY, 132 :1639-1649 [Details]
1998 'Expression and tissue distribution of the mRNAs encoding the human thromboxane A2 receptor (TP) alpha and beta isoforms'
Miggin, SM and Kinsella, BT. (1998) 'Expression and tissue distribution of the mRNAs encoding the human thromboxane A2 receptor (TP) alpha and beta isoforms'. BIOCHIMICA ET BIOPHYSICA ACTA, 1425 :543-59 [Details]

Book Chapters

  Year Publication
2011 'Toll-like receptor adaptor proteins'
Shevlin, Enda and Miggin, Sinéad M. (2011) 'Toll-like receptor adaptor proteins' In: Encyclopedia of Signaling Molecules. Springer. [Details]
2009 'Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells'
Siednienko, J. and Miggin, S.M. (2009) 'Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells' In: Methods in Molecular Biology, Vol. 517. New Jersey: Humana Press Inc. [Details]