Dr Paul Dowling

Biology

Lecturer
International Coordinator

Callan
1st
2.35
(01) 7086368

Biography

Career profile (Education and Employment): • BSc (University College Dublin, Pharmacology Dept.) • PhD (NUI Maynooth, Biology Dept.) Academic Experience: • Lecturing, Practical class supervision, Workgroup supervision: University College Dublin, Pharmacology Dept. 2001-2002 • Lecturing, Practical class supervision, Workgroup supervision: NUI Maynooth, Biology Dept. 2002-2003 • Lecturing, Supervision of post-graduate students, Practical class supervision, Workgroup supervision: Dublin City University, NICB. 2004-2013 • Lecturing, Practical class supervision, Workgroup supervision: NUI Maynooth, Biology Dept. 2013- Research Experience: • 3 years University College Dublin, Pharmacology Dept. (Muscular Dystrophy) • 1 year NUI Maynooth, Biology Dept. (Muscular Dystrophy; Myasthenia Gravis) • 9 years Dublin City University, NICB. (Cancer; Diabetes; Muscle biology) Research Funding & Awards: Ongoing Research Support: Enterprise Ireland CF 2012 2005 - 01/10/2012-30/09/2013 “New Serum Biomarkers for Early Detection of Lung Cancer”. Role: PI, Award: €106,493 Mater Hospital Foundation, Serum protein biomarkers for drug response in Multiple Myeloma patients 2006-2013 Role: Joint PI, Award: €230,000 Completed Research Support Enterprise Ireland PC/2008/0368 - 01/01/2009-31/12/2010 “Validation of a panel of serum biomarkers for use in early detection and monitoring of response to therapy in squamous cell carcinoma of the lung”. Role: Joint PI, Award: €100,000 Currently supervising: 5 Andrew McCann (PhD, DCU): Analysis of conditioned media from cancer cell lines as a source of candidate biomarkers for early detection and therapeutic monitoring. Catriona Hayes (PhD, Mater/Dana Farber): Analysis of the interactions between plasma cells with the micro-environment and its role in Bortezomib resistance. MinYuen Teo (MD, St Vincents/Tallaght): Identification of biomarkers for the early detection of recurrence in colorectal cancer patients. Kay Reen Ting (MD, Mater): Investigating biomarkers predicting response to Bortezomib in Multiple Myeloma patients. Benvon Moran (MD, St James): Examining molecular mechanisms involved in the progression of malignant melanoma. Previously supervised: 1 Rajesh Rajpal (PhD, Mater): Identification of novel protein biomarkers for predicting response to thalidomide-based therapy in newly diagnosed multiple myeloma patients (2007-2011) Damian Pollard (PhD, DCU): Identification of biomarkers for the early detection and therapeutic monitoring in lung cancer (2007-2013).

Book Chapters

  Year Publication
2011 'Clinical Research and Applications: Sample Preparation Techniques for Cancer Proteomics'
Paul Dowling, Martin Clynes, and Paula Meleady (2011) 'Clinical Research and Applications: Sample Preparation Techniques for Cancer Proteomics' In: Sample Preparation in Biological Mass Spectrometry. Springer. [Details]

Peer Reviewed Journals

  Year Publication
2014 '7B7: a novel antibody directed against the Ku70/Ku80 heterodimer blocks invasion in pancreatic and lung cancer cells'
O'Sullivan D, Henry M, Joyce H, Walsh N, Auley EM, Dowling P, Swan N, Moriarty M, Barnham P, Clynes M, Larkin A (2014) '7B7: a novel antibody directed against the Ku70/Ku80 heterodimer blocks invasion in pancreatic and lung cancer cells'. TUMOR BIOLOGY, 35 :6983-6997 [DOI] [IR Link] [Details]
2014 'New pathobiochemical insights into dystrophinopathy from the proteomics of senescent mdx mouse muscle'
Holland A, Dowling P, Ohlendieck K (2014) 'New pathobiochemical insights into dystrophinopathy from the proteomics of senescent mdx mouse muscle'. To be added, 6 [DOI] [IR Link] [Details]
2014 'Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry'
Holland A, Schmitt-John T, Dowling P, Meleady P, Henry M, Clynes M, Ohlendieck K (2014) 'Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry'. BIOSCIENCE REPORTS, 34 :331-343 [DOI] [IR Link] [Details]
2014 'Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia'
Jockusch H, Holland A, Staunton L, Schmitt-John T, Heimann P, Dowling P, Ohlendieck K (2014) 'Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia'. PROTEOMICS, 14 :839-852 [DOI] [IR Link] [Details]
2014 'PP2A inhibition overcomes acquired resistance to HER2 targeted therapy'
McDermott MS, Browne BC, Conlon NT, O'Brien NA, Slamon DJ, Henry M, Meleady P, Clynes M, Dowling P, Crown J, O'Donovan N (2014) 'PP2A inhibition overcomes acquired resistance to HER2 targeted therapy'. To be added, 13 [DOI] [IR Link] [Details]
2014 'Mass Spectrometry-Based Identification of Muscle-Associated and Muscle-Derived Proteomic Biomarkers of Dystrophinopathies'
Paul Dowling, Ashling Holland, Kay Ohlendieck1 (2014) 'Mass Spectrometry-Based Identification of Muscle-Associated and Muscle-Derived Proteomic Biomarkers of Dystrophinopathies'. To be added, 1 :15-40 [DOI] [IR Link] [Details]
2013 'Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia'
Jockusch H, Holland A, Staunton L, Schmitt-John T, Heimann P, Dowling P, Ohlendieck K. (2013) 'Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia'. PROTEOMICS, Published online ahead of print [Details]
2013 'Proteomic profiling of cardiomyopathic tissue from the aged mdx model of Duchenne muscular dystrophy reveals a drastic decrease in laminin, nidogen and annexin'
Holland A, Dowling P, Zweyer M, Swandulla D, Henry M, Clynes M, Ohlendieck K. (2013) 'Proteomic profiling of cardiomyopathic tissue from the aged mdx model of Duchenne muscular dystrophy reveals a drastic decrease in laminin, nidogen and annexin'. PROTEOMICS, 13 :2312-2323 [IR Link] [Details]
2012 'Analysis of acute phase proteins, AHSG, C3, CLI, HP and SAA reveals distinctive expression patterns associated with breast, colorectal and lung cancer'
Dowling P, Clarke C, Hennessy K, Torralbo-Lopez B, Ballot J, Crown J, Kiernan I, O'Byrne KJ, Kennedy MJ, Lynch V, Clynes M. (2012) 'Analysis of acute phase proteins, AHSG, C3, CLI, HP and SAA reveals distinctive expression patterns associated with breast, colorectal and lung cancer'. INTERNATIONAL JOURNAL OF CANCER, 131 :911-923 [IR Link] [Details]
2012 'The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cancer cell lines'
O'Connell K, Prencipe M, O'Neill A, Corcoran C, Rani S, Henry M, Dowling P, Meleady P, O'Driscoll L, Watson W, O'Connor R. (2012) 'The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cancer cell lines'. PROTEOMICS, 12 :2115-2116 [IR Link] [Details]
2011 'Conditioned media from cell lines: A complementary model to clinical specimens for the discovery of disease-specific biomarkers'
Dowling P, Clynes M. (2011) 'Conditioned media from cell lines: A complementary model to clinical specimens for the discovery of disease-specific biomarkers'. PROTEOMICS, 4 :794-804 [Details]
2011 'RNAi knockdown of Hop (Hsp70/Hsp90 organising protein) decreases invasion via MMP-2 down regulation'
Walsh N, Larkin A, Swan N, Conlon K, Dowling P, McDermott R, Clynes M. (2011) 'RNAi knockdown of Hop (Hsp70/Hsp90 organising protein) decreases invasion via MMP-2 down regulation'. CANCER LETTERS, 306 :180-189 [Details]
2011 '2D-DIGE analysis of phospho-enriched fractions from dasatinib treated melanoma cell lines'
Eustace AJ*, Dowling P*, Henry M, Doolan P, Meleady P, Clynes M, Crown J, O'Donovan N. *Joint authors (2011) '2D-DIGE analysis of phospho-enriched fractions from dasatinib treated melanoma cell lines'. JOURNAL OF PROTEOMICS, 4 :490-501 [Details]
2011 '2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to fourteen consecutive days of endurance exercise training'
Egan B*, Dowling P*, OConnor P, Henry M, Meleady P, Zierath J, OGorman D. *Joint authors (2011) '2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to fourteen consecutive days of endurance exercise training'. PROTEOMICS, 11 :1413-1428 [Details]
2011 'A novel panel of protein biomarkers for predicting response to thalidomide-based therapy in newly diagnosed Multiple Myeloma patients'
Rajpal R*, Dowling P*, Meiller J, Clarke C, Murphy W, OConnor R, Kell M, Mitsiades C, Richardson P, Anderson K, Clynes M, OGorman P. *Joint authors (2011) 'A novel panel of protein biomarkers for predicting response to thalidomide-based therapy in newly diagnosed Multiple Myeloma patients'. PROTEOMICS, 8 :1391-1402 [Details]
2010 'Recent advances in clinical proteomics using mass spectrometry'
Dowling P, Meleady P, Henry M, Clynes M. (2010) 'Recent advances in clinical proteomics using mass spectrometry'. BIOANALYSIS, 2 :1609-1615 [Details]
2009 'Identification of pancreatic cancer invasion-related proteins by proteomic analysis'
Walsh N, ODonovan N, Henry M, Meleady P, Clynes M, Dowling P. (2009) 'Identification of pancreatic cancer invasion-related proteins by proteomic analysis'. PROTEOME SCIENCE, 7 [Details]
2009 'MiRNA-29a regulates the expression of numerous proteins and reduces the invasiveness and proliferation of human carcinoma cell lines'
Muniyappa MK, Dowling P, Henry M, Meleady P, Doolan P, Gammell P, Clynes M, Barron N. (2009) 'MiRNA-29a regulates the expression of numerous proteins and reduces the invasiveness and proliferation of human carcinoma cell lines'. EUROPEAN JOURNAL OF CANCER, 45 :3104-3118 [Details]
2008 'Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells'
Walsh N*, Dowling P*, O'Donovan N, Henry M, Meleady P, Clynes M. *Joint authors (2008) 'Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells'. JOURNAL OF PROTEOMICS, 71 :561-571 [Details]
2008 'Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry'
Kumar N, Maurya P, Gammell P, Dowling P, Clynes M, Meleady P. (2008) 'Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry'. BIOTECHNOLOGY PROGRESS, 24 :273-278 [Details]
2008 'Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis'
Dowling P, Wormald R, Meleady P, Henry M, Curran A, Clynes M. (2008) 'Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis'. JOURNAL OF PROTEOMICS, 71 :168-175 [Details]
2008 'Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction'
Dowling P, Shields W, Rani S, Meleady P, Henry M, Jeppesen P, O'Driscoll L, Clynes M. (2008) 'Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction'. ELECTROPHORESIS, 29 :4141-4149 [Details]
2008 'Membrane and membrane-associated proteins involved in the aggressive phenotype displayed by highly invasive cancer cells'
Dowling P, Walsh N, Clynes M. (2008) 'Membrane and membrane-associated proteins involved in the aggressive phenotype displayed by highly invasive cancer cells'. PROTEOMICS, 8 :4054-4065 [Details]
2007 'Two-dimensional difference gel electrophoresis of the Lung Squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of 10 specific proteins'
Dowling P, ODriscoll L, Meleady P, Henry M, Roy S, Ballot J, Moriarty M, Crown J, Clynes M. (2007) 'Two-dimensional difference gel electrophoresis of the Lung Squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of 10 specific proteins'. ELECTROPHORESIS, 28 :4302-4310 [Details]
2007 'Proteomic analysis of isolated membrane fractions from superinvasive cancer cells'
Dowling P, Meleady P, Dowd A, Henry M, Glynn S, Clynes M. (2007) 'Proteomic analysis of isolated membrane fractions from superinvasive cancer cells'. BIOCHIMICA ET BIOPHYSICA ACTA, 1774 :93-101 [Details]
2007 'Purification and identification of a 7.6-kDa protein in media conditioned by superinvasive cancer cells'
Dowling P, Maurya P, Meleady P, Glynn SA, Dowd AJ, Henry M, Clynes M. (2007) 'Purification and identification of a 7.6-kDa protein in media conditioned by superinvasive cancer cells'. ANTICANCER RESEARCH, 27 :1309-1317 [Details]
2006 'Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle'
Doran P, Dowling P, Donoghue P, Buffini M, Ohlendieck K. (2006) 'Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle'. BIOCHIMICA ET BIOPHYSICA ACTA, 1764 :773-785 [Details]
2006 'Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress'
Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M. (2006) 'Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress'. PROTEOMICS, 6 :6578-6587 [Details]
2006 'Proteome analysis of the dystrophin-deficient MDX diaphragm reveals a drastic increase in the heat shock protein cvHSP'
Doran P, Martin G, Dowling P, Jockusch H, Ohlendieck K. (2006) 'Proteome analysis of the dystrophin-deficient MDX diaphragm reveals a drastic increase in the heat shock protein cvHSP'. PROTEOMICS, 6 :4610-4621 [Details]
2005 'Differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulation'
Donoghue P, Doran P, Dowling P, Ohlendieck K. (2005) 'Differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulation'. BIOCHIMICA ET BIOPHYSICA ACTA, 1752 :166-176 [Details]
2004 'Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy'
Dowling P, Doran P, Ohlendieck K. (2004) 'Drastic reduction of sarcalumenin in Dp427 (dystrophin of 427 kDa)-deficient fibres indicates that abnormal calcium handling plays a key role in muscular dystrophy'. BIOCHEMICAL JOURNAL, 379 :479-488 [Details]
2004 'Naturally protected muscle phenotypes: Development of novel treatment strategies for Duchenne Muscular Dystrophy'
Dowling P, Doran P, Ohlendieck K. (2004) 'Naturally protected muscle phenotypes: Development of novel treatment strategies for Duchenne Muscular Dystrophy'. BASIC AND APPLIED MYOLOGY, 14 :169-178 [Details]
2004 'Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle'
Doran P, Dowling P, Lohan J, McDonnell K, Poetsch S, Ohlendieck K. (2004) 'Subproteomics analysis of Ca+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle'. EUROPEAN JOURNAL OF BIOCHEMISTRY, 271 :3943-3952 [Details]
2003 'Comparative analysis of Dp427-deficient mdx tissues shows that the milder dystrophic phenotype of extraocular and toe muscle fibres is associated with a persistent expression of beta-dystroglycan'
Dowling P, Lohan J, Ohlendieck K. (2003) 'Comparative analysis of Dp427-deficient mdx tissues shows that the milder dystrophic phenotype of extraocular and toe muscle fibres is associated with a persistent expression of beta-dystroglycan'. EUROPEAN JOURNAL OF CELL BIOLOGY, 82 :222-230 [Details]
2002 'Distal mdx muscle groups exhibiting up-regulation of utrophin and rescue of dystrophin-associated glycoproteins exemplify a protected phenotype in muscular dystrophy'
Dowling P, Culligan K, Ohlendieck K. (2002) 'Distal mdx muscle groups exhibiting up-regulation of utrophin and rescue of dystrophin-associated glycoproteins exemplify a protected phenotype in muscular dystrophy'. NATURWISSENSCHAFTEN, 89 :75-78 [Details]
2002 'Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle'
Culligan K, Banville N, Dowling P, Ohlendieck K. (2002) 'Drastic reduction of calsequestrin-like proteins and impaired calcium binding in dystrophic mdx muscle'. JOURNAL OF APPLIED PHYSIOLOGY, 92 :435-445 [Details]
2001 'Brain dystrophin-glycoprotein complex: persistent expression of beta-dystroglycan, impaired oligomerization of Dp71 and up-regulation of utrophins in animal models of muscular dystrophy'
Culligan K, Glover L, Dowling P, Ohlendieck K. (2001) 'Brain dystrophin-glycoprotein complex: persistent expression of beta-dystroglycan, impaired oligomerization of Dp71 and up-regulation of utrophins in animal models of muscular dystrophy'. BMC CELL BIOLOGY, 2 [Details]

Book Review

  Year Publication
2007 Proteomic approaches for serum biomarker discovery in cancer.
Maurya P, Meleady P, Dowling P, Clynes M. (2007) Proteomic approaches for serum biomarker discovery in cancer. Book Review [Details]
2007 Drug resistance in cancer Searching for mechanisms, markers and therapeutic agents.
O'Connor R, Clynes M, Dowling P, O'Donovan N, O'Driscoll L. (2007) Drug resistance in cancer Searching for mechanisms, markers and therapeutic agents. Book Review [Details]