Dr Emmanuelle Graciet

    Biology, Human Health Institute

    Associate Professor

    Biosciences & Electronic Engineering Facility
    B1.25
    (01) 708 6255

    Biography

    2000: MSc in Plant Molecular and Cellular Physiology (Institut National Agronomique Paris-Grignon (now AgroParisTech)), France
    2003: PhD in Biochemistry (University Pierre and Marie Curie (Paris 6)), France
    2003-2006: postdoctoral fellow (Caltech), USA
    2006-2009: postdoctoral fellow (Trinity College Dublin)
    2009-2013: SFI-SIRG principal investigator (Trinity College Dublin)
    2014-2020: Assistant Professor/Lecturer (Maynooth University)
    From 2020: Associate Professor (Maynooth University)

    Research Interests

    Research in the Plant Biochemistry Lab focuses on the role of protein degradation mediated by the ubiquitin system in the regulation of plant responses to biotic and abiotic stresses and to combinations of stresses, with a focus on waterlogging/hypoxia and pathogens. 

    For this work, we use the model plant Arabidopsis thaliana, but also crops such as barley (Hordeum vulgare) and oilseed rape (Brassica napus), in combination with biochemical, molecular, genetic and genomics approaches. More specifically, we aim at understanding how the nature of the N-terminal residue of proteins affects their stability and participates to the regulation of plant-pathogen interactions.

    Another aspect of our work aims at understanding how plants respond to combinations of environmental stresses, with a focus on waterlogging (and the resulting hypoxia) and pathogens. The Plant Biochemistry Lab is a member of:

    • the Virtual Irish Centre for Crop Improvement (VICCI). Additional details available at: http://vicci.ie/
    • the Sustainable Ecosystems Research Group at Maynooth University


    Peer Reviewed Journal

    Year Publication
    2024 Brian C. Mooney; Catherine M. Doorly; Melissa Mantz; Pablo Garcia; Pitter Huesgen; Emmanuelle Graciet (2024) 'Hypoxia represses pattern-triggered immune responses in Arabidopsis'. PLANT PHYSIOLOGY, .
    2024 Garcia, P.; Singh, S.; Graciet, E. (2024) 'New Insights into the Connections between Flooding/Hypoxia Response and Plant Defenses against Pathogens'. Plants (Basel, Switzerland), 13 . [DOI]
    2023 Zhang, H; Rundle, C; Winter, N; Miricescu, A; Mooney, BC; Bachmair, A; Graciet, E; Theodoulou, FL (2023) 'BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition'. The Plant cell, . [DOI]
    2023 Brazel, A.J.; Graciet, E. (2023) 'Complexity of Abiotic Stress Stimuli: Mimicking Hypoxic Conditions Experimentally on the Basis of Naturally Occurring Environments'. Methods in molecular biology (Clifton, N.J.), 2642 . [Link] [DOI]
    2023 Miricescu, A.; Brazel, A.J.; Beegan, J.; Wellmer, F.; Graciet, E. (2023) 'Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response'. Plant Direct, 7 . [Link] [DOI] [Full-Text]
    2022 Byrne T.; Grant J.; Kock-Appelgren P.; Förster L.; Michel T.; Miricescu A.; Thomas W.T.B.; Graciet E.; Spink J.; Ng C.K.Y.; Barth S. (2022) 'Improving phenotyping in winter barley cultivars towards waterlogging tolerance by combining field trials under natural conditions with controlled growth condition experiments'. European Journal of Agronomy, 133 . [DOI] [Full-Text]
    2021 Doorly C.M.; Graciet E. (2021) 'Lessons from comparison of hypoxia signaling in plants and mammals'. Plants (Basel, Switzerland), 10 (5). [DOI] [Full-Text]
    2021 Miricescu A.; Byrne T.; Doorly C.M.; Ng C.K.Y.; Barth S.; Graciet E. (2021) 'Experimental comparison of two methods to study barley responses to partial submergence'. Plant Methods, 17 (1). [DOI] [Full-Text]
    2021 Mooney BC; Mantz M; Graciet E; Huesgen PF; (2021) 'Cutting the line: manipulation of plant immunity by bacterial type III effector proteases'. Journal of Experimental Botany, . [DOI] [Full-Text]
    2020 Mooney B.C.; Graciet E. (2020) 'A simple and efficient Agrobacterium-mediated transient expression system to dissect molecular processes in Brassica rapa and Brassica napus'. Plant Direct, 4 (7). [DOI] [Full-Text]
    2019 Goslin K; Eschen-Lippold L; Naumann C; Linster E; Sorel M; Klecker M; de Marchi R; Kind A; Wirtz M; Lee J; Dissmeyer N; Graciet E; (2019) 'Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease'. Plant Physiology, 180 :2272-2289. [DOI] [Full-Text]
    2018 Miricescu A; Goslin K; Graciet E; (2018) 'Ubiquitylation in plants: signaling hub for the integration of environmental signals'. Journal of Experimental Botany, 69 (19):4511-4527. [DOI] [Full-Text]
    2018 Dissmeyer N.; Rivas S.; Graciet E. (2018) 'Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway'. New Phytologist, 218 (3):929-935. [DOI] [Full-Text]
    2017 Dissmeyer N; Graciet E; Holdsworth MJ; Gibbs DJ; (2017) 'N-term 2017: Proteostasis via the N-terminus'. Trends in Biochemical Sciences, 44 :293-295. [DOI] [Full-Text]
    2017 Goslin K; Zheng B; Serrano-Mislata A; Rae L; Ryan PT; Kwaśniewska K; Thomson B; O'Maoileidigh D; Madueno F; Wellmer F; Graciet E; (2017) 'Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation'. Plant Physiology, 174 :1097-1109. [DOI] [Full-Text]
    2017 Serrano-Mislata A; Goslin K; Zheng B; Rae L; Wellmer F; Graciet E; Madueño F; (2017) 'Regulatory interplay between LEAFY, APETALA1/CAULIFLOWER and TERMINAL FLOWER1: New insights into an old relationship'. Plant Signaling and Behavior, 12 . [DOI] [Full-Text]
    2016 de Marchi R; Sorel M; Mooney B; Fudal I; Goslin K; Kwaśniewska K; Ryan PT; Pfalz M; Kroymann J; Pollmann S; Feechan A; Wellmer F; Rivas S; Graciet E; (2016) 'The N-end rule pathway regulates pathogen responses in plants'. Scientific Reports, 6 . [DOI] [Full-Text]
    2015 O'Maoileidigh, DS; Thomson, B; Raganelli, A; Wuest, SE; Ryan, PT; Kwasniewska, K; Carles, CC; Graciet, E; Wellmer, F (2015) 'Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations'. Plant Journal, 83 :344-358. [DOI] [Full-Text]
    2015 Ryan, PT; O'Maoileidigh, DS; Drost, HG; Kwasniewska, K; Gabel, A; Grosse, I; Graciet, E; Quint, M; Wellmer, F (2015) 'Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation'. BMC Genomics, 16 . [DOI] [Full-Text]
    2014 D. Ó’Maoiléidigh, E. Graciet and F. Wellmer (2014) 'Gene networks controlling flower development'. New Phytologist, 201 :16-30. [Full-Text]
    2014 Wellmer, F; Graciet, E; Riechmann, JL (2014) 'Specification of floral organs in Arabidopsis'. Journal of Experimental Botany, 65 :1-9. [DOI] [Full-Text]
    2013 Piatkov K; Graciet E; Varshavsky A; (2013) 'Ubiquitin reference technique and its use in ubiquitin-lacking prokaryotes'. PLoS ONE, 8 (6). [DOI] [Full-Text]
    2013 D. Ó’Maoiléidigh, S. E. Wuest, L. Rae, P. T. Ryan, K. Kwaśniewska, P. Das, A. Lohan, B. Loftus, E. Graciet*, and F. Wellmer (2013) 'Interplay of B and C function regulators in the control of floral organ development'. Plant Cell, 25 :2482-2503. [Full-Text]
    2012 S. E. Wuest, D. ӒMaoilidigh, L. Rae, K. Kwaśniewska, A. Raganelli, K. Hanczaryk, A. Lohan, B. Loftus, E. Graciet, and F. Wellmer (2012) 'Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 109 :13452-13457. [Full-Text]
    2010 E. Graciet*, F. Mesiti and F. Wellmer* (2010) 'Structure and evolutionary conservation of the plant N-end rule pathway'. Plant Journal, 61 :741-751. [Full-Text]
    2010 E. Graciet and F. Wellmer (2010) 'The plant N-end rule pathway: structure and functions'. Trends in Plant Science, 15 :447-453. [Full-Text]
    2009 E. Graciet*, F. Walter, D. OMaoilidigh, S. Pollmann, E. M. Meyerowitz, A. Varshavsky and F. Wellmer* (2009) 'The N-end rule pathway controls multiple functions during Arabidopsis shoot and leaf development'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 106 :13618-13623. [Full-Text]
    2006 Lebreton S; Andreescu S; Graciet E; Gontero B; (2006) 'Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii. Functional consequences for glyceraldehyde-3-phosphate dehydrogenase'. FEBS Journal, 273 (14):3358-3369. [DOI] [Full-Text]
    2006 E. Graciet, R-G. Hu, K. Piatkov, J.H. Rhee, E. M. Schwarz and A. Varshavsky (2006) 'Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 103 :3078-3083. [Full-Text]
    2005 Delobel A.; Graciet E.; Andreescu S.; Gontero B.; Halgand F.; Laprévote O. (2005) 'Rapid Communications in Mass Spectrometry'. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 19 (22):3379-3388. [DOI] [Full-Text]
    2004 E. Graciet, S. Lebreton, and B. Gontero (2004) 'Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex'. Journal of Experimental Botany, 55 :1245-1254. [Full-Text]
    2004 E. Graciet, G. Mulliert, S. Lebreton, and B. Gontero (2004) 'Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation'. European Journal of Biochemistry, 271 :4737-4744. [Full-Text]
    2003 Graciet, E., Gans, P., Wedel, N., Lebreton, S., Camadro, J.-M., Gontero, B. (2003) 'The small protein CP12: A protein linker for supramolecular complex assembly'. 42 (27):8163-8170. [Link] [DOI] [Full-Text]
    2003 S. Lebreton, E. Graciet, and B. Gontero (2003) 'Modulation, via protein-protein interactions, of glyceraldehyde-3-phosphate dehydrogenase activity through redox phosphoribulokinase regulation'. Journal of Biological Chemistry, 278 :12078-12084. [Full-Text]
    2003 E. Graciet, S. Lebreton, J-M. Camadro, and B. Gontero (2003) 'Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for conformation changes upon association with the small protein CP12'. European Journal of Biochemistry, 270 :129-136. [Full-Text]
    2000 Wessel, P.M., Graciet, E., Douce, R., Dumas, R. (2000) 'Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments'. 39 (49):15136-15143. [Link] [DOI] [Full-Text]

    Book Chapter

    Year Publication
    2017 Thomson B; Graciet E; Wellmer F; (2017) 'Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis' In: Methods in molecular biology. USA : Clifton, N.J. [DOI]
    2016 Stewart D; Graciet E; Wellmer F; (2016) 'Molecular and regulatory mechanisms controlling floral organ development' In: [DOI]
    2014 Graciet E; O'Maoiléidigh DS; Wellmer F; (2014) 'Next-generation sequencing applied to flower development: ChIP-Seq' In: Methods in molecular biology. USA : Clifton N.J. [DOI] [Full-Text]
    2014 Wellmer F; Bowman JL; Davies B; Ferrándiz C; Fletcher JC; Franks RG; Graciet E; Gregis V; Ito T; Jack TP; Jiao Y; Kater MM; Ma H; Meyerowitz EM; Prunet N; Riechmann JL; (2014) 'Flower development: open questions and future directions' In: Methods in Molecular Biology - Flower Development. USA : Springer. [DOI]
    2014 O'Maoileidigh, DS; Graciet, E; Wellmer, F (2014) 'Genetic Control of Arabidopsis Flower Development' In: MOLECULAR GENETICS OF FLORAL TRANSITION AND FLOWER DEVELOPMENT. LONDON : ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD. [DOI]

    Editorial

    Year Publication
    2022 Willems P.; Huesgen P.F.; Finkemeier I.; Graciet E.; Meinnel T.; Van Breusegem F. (2022) Editorial: Plant protein termini: Their generation, modification and function. [Editorial] [DOI]
    Certain data included herein are derived from the © Web of Science (2024) of Clarivate. All rights reserved.

    Professional Associations

    Description Function From / To
    Irish Plant Scientists Association Member -
    American Society of Plant Biologists Member -
    Society of Experimental Biology (SEB) Member -

    Honors and Awards

    Date Title Awarding Body
    01/01/2007 EMBO Long Term Fellowship
    01/01/2004 HFSP Long-Term Postdoctoral Fellowship

    Committees

    Committee Function From / To
    Academic Council Member 01/09/2019 - 30/06/2022
    Second year Biology Coordinator Coordinator 01/09/2020 -
    Departmental Teaching & Curriculum committee Member 01/06/2020 -
    Departmental Research Committee (Coordinator) Coordinator 01/10/2018 - 31/10/2022
    Faculty Research Committee Committee member 01/09/2015 - 30/06/2019
    University Campus Life Committee Member 01/12/2019 - 31/07/2022
    Biological Safety Committee Member 01/09/2016 -
    Departmental Academic Integrity group (Coordinator) Coordinator 01/09/2024 -
    Faculty of Science and Engineering (Secretary) Secretary 01/09/2024 -
    Maynooth University Green Labs committee (secretary) Secretary 01/06/2024 -

    Employment

    Employer Position From / To
    Maynooth University Lecturer 02/01/2014 -
    Trinity College Dublin/SFI SIRG Principal Investigator 01/10/2009 - 31/12/2013
    Trinity College Dublin Postdoctoral Research Fellow 01/04/2009 - 30/09/2009
    EMBO fellowship EMBO postdoctoral fellow 01/04/2007 - 31/03/2009
    California Insitute of Technology Postdoctoral research fellow (HFSP fellowship) 01/04/2004 - 31/03/2007
    California Insitute of Technology Postdoctoral Research Fellow 01/08/2003 - 31/03/2004

    Education

    Start date Institution Qualification Subject
    Pierre and Marie Curie University (Paris 6) PhD
    Institut National Agronomique Paris-Grignon MSc
    Institut National Agronomique Paris-Grignon Diplome d'Agronomie Generale Agronomy

    Languages

    Language Reading Writing Speaking
    German Functional Functional Functional
    English Fluent Fluent Fluent
    Portuguese Functional Basic Functional
    Spanish Fluent Functional Fluent
    French Fluent Fluent Fluent

    Teaching Interests

    My primary teaching interests are plant biology and biochemistry.

    I lecture in the following modules:
    - BI201 - Biochemistry
    - BI305 - Literature review, including tutorials
    - BI306 - Plant Responses to the Environment
    - BI309 - General Methodology
    - BI440 - Control of Protein Activity and Abundance
    - BI425 - BioPharma - producing human therapeutics in plants

    I am also the second year coordinator and MH201 Deputy course coordinator for the Biology Department.


    Recent Students

    Graduation date Name Degree
    2022 Brian Mooney PhD
    2022 Tara O'Connor PhD
    2019 Alexandra Miricescu PhD
    2019 Kevin Goslin PhD
    2014 Katarzyna Hanczaryk PhD
    2010 Francesca Mesiti MSc
    2009 Franziska Walter MSc