Dr Emmanuelle Graciet

    Biology, Human Health Institute

    Associate Professor

    Biosciences & Electronic Engineering Facility
    B1.25
    (01) 708 6255

    Biography

    2000: MSc in Plant Molecular and Cellular Physiology (Institut National Agronomique Paris-Grignon (now AgroParisTech)), France
    2003: PhD in Biochemistry (University Pierre and Marie Curie (Paris 6)), France
    2003-2006: postdoctoral fellow (Caltech), USA
    2006-2009: postdoctoral fellow (Trinity College Dublin)
    2009-2013: SFI-SIRG principal investigator (Trinity College Dublin)
    2014-2020: Assistant Professor/Lecturer (Maynooth University)
    From 2020: Associate Professor (Maynooth University)

    Research Interests

    Research in the Plant Biochemistry Lab focuses on the role of protein degradation mediated by the ubiquitin system in the regulation of plant responses to biotic and abiotic stresses.

    For this work, we use the model plant Arabidopsis thaliana, but also crops such as barley and Brassica rapa (turnip), in combination with biochemical, molecular, genetic and genomics approaches. More specifically, we aim at understanding how the nature of the N-terminal residue of proteins affects their stability and participates to the regulation of plant-pathogen interactions. Another aspect of the work aims at gaining further insights into the role of protein degradation in the regulation of transcription in the context of biotic and abiotic stresses.

    The Plant Biochemistry Lab is a member of the Virtual Irish Centre for Crop Improvement (VICCI). Additional details available at: http://vicci.ie/

    Peer Reviewed Journals

      Year Publication
    2021 'Cutting the line: manipulation of plant immunity by bacterial type III effector proteases'
    Mooney BC;Mantz M;Graciet E;Huesgen PF; (2021) 'Cutting the line: manipulation of plant immunity by bacterial type III effector proteases'. Journal of Experimental Botany, [DOI] [Details]
    2021 'Experimental comparison of two methods to study barley responses to partial submergence'
    Miricescu A., Byrne T., Doorly C.M., Ng C., Barth S., Graciet E. (2021) 'Experimental comparison of two methods to study barley responses to partial submergence'. Plant Methods, [Details]
    2020 'A simple and efficient Agrobacterium-mediated transient expression system to dissect molecular processes in Brassica rapa and Brassica napus'
    Mooney B.C.;Graciet E. (2020) 'A simple and efficient Agrobacterium-mediated transient expression system to dissect molecular processes in Brassica rapa and Brassica napus'. Plant Direct, 4 (7) [DOI] [Details]
    2019 'Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease'
    Goslin K;Eschen-Lippold L;Naumann C;Linster E;Sorel M;Klecker M;de Marchi R;Kind A;Wirtz M;Lee J;Dissmeyer N;Graciet E; (2019) 'Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease'. Plant Physiology, 180 :2272-2289 [DOI] [full-text] [Details]
    2018 'Ubiquitylation in plants: signaling hub for the integration of environmental signals'
    Miricescu A;Goslin K;Graciet E; (2018) 'Ubiquitylation in plants: signaling hub for the integration of environmental signals'. Journal of Experimental Botany, 69 (19):4511-4527 [DOI] [full-text] [Details]
    2018 'Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway'
    Dissmeyer N.;Rivas S.;Graciet E. (2018) 'Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway'. New Phytologist, 218 (3):929-935 [DOI] [full-text] [Details]
    2017 'Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation'
    Goslin K;Zheng B;Serrano-Mislata A;Rae L;Ryan PT;Kwaśniewska K;Thomson B;O'Maoileidigh D;Madueno F;Wellmer F;Graciet E; (2017) 'Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation'. Plant Physiology, 174 :1097-1109 [DOI] [full-text] [Details]
    2017 'N-term 2017: Proteostasis via the N-terminus'
    Dissmeyer N;Graciet E;Holdsworth MJ;Gibbs DJ; (2017) 'N-term 2017: Proteostasis via the N-terminus'. Trends in Biochemical Sciences, 44 :293-295 [DOI] [full-text] [Details]
    2017 'Regulatory interplay between LEAFY, APETALA1/CAULIFLOWER and TERMINAL FLOWER1: New insights into an old relationship'
    Serrano-Mislata A;Goslin K;Zheng B;Rae L;Wellmer F;Graciet E;Madueño F; (2017) 'Regulatory interplay between LEAFY, APETALA1/CAULIFLOWER and TERMINAL FLOWER1: New insights into an old relationship'. Plant Signaling and Behavior, 12 [DOI] [full-text] [Details]
    2016 'The N-end rule pathway regulates pathogen responses in plants'
    de Marchi R;Sorel M;Mooney B;Fudal I;Goslin K;Kwaśniewska K;Ryan PT;Pfalz M;Kroymann J;Pollmann S;Feechan A;Wellmer F;Rivas S;Graciet E; (2016) 'The N-end rule pathway regulates pathogen responses in plants'. Scientific Reports, 6 [DOI] [full-text] [Details]
    2015 'Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations'
    O'Maoileidigh, DS;Thomson, B;Raganelli, A;Wuest, SE;Ryan, PT;Kwasniewska, K;Carles, CC;Graciet, E;Wellmer, F (2015) 'Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations'. Plant Journal, 83 :344-358 [DOI] [full-text] [Details]
    2015 'Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation'
    Ryan, PT;O'Maoileidigh, DS;Drost, HG;Kwasniewska, K;Gabel, A;Grosse, I;Graciet, E;Quint, M;Wellmer, F (2015) 'Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation'. BMC Genomics, 16 [DOI] [full-text] [Details]
    2014 'Gene networks controlling flower development'
    D. Ó’Maoiléidigh, E. Graciet and F. Wellmer (2014) 'Gene networks controlling flower development'. New Phytologist, 201 :16-30 [full-text] [Details]
    2014 'Specification of floral organs in Arabidopsis'
    Wellmer, F;Graciet, E;Riechmann, JL (2014) 'Specification of floral organs in Arabidopsis'. Journal of Experimental Botany, 65 :1-9 [DOI] [full-text] [Details]
    2013 'Ubiquitin reference technique and its use in ubiquitin-lacking prokaryotes'
    Piatkov K;Graciet E;Varshavsky A; (2013) 'Ubiquitin reference technique and its use in ubiquitin-lacking prokaryotes'. PLoS ONE, 8 (6) [DOI] [full-text] [Details]
    2013 'Interplay of B and C function regulators in the control of floral organ development'
    D. Ó’Maoiléidigh, S. E. Wuest, L. Rae, P. T. Ryan, K. Kwaśniewska, P. Das, A. Lohan, B. Loftus, E. Graciet*, and F. Wellmer (2013) 'Interplay of B and C function regulators in the control of floral organ development'. Plant Cell, 25 :2482-2503 [full-text] [Details]
    2012 'Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA'
    S. E. Wuest, D. ӒMaoilidigh, L. Rae, K. Kwaśniewska, A. Raganelli, K. Hanczaryk, A. Lohan, B. Loftus, E. Graciet, and F. Wellmer (2012) 'Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 109 :13452-13457 [full-text] [Details]
    2010 'Structure and evolutionary conservation of the plant N-end rule pathway'
    E. Graciet*, F. Mesiti and F. Wellmer* (2010) 'Structure and evolutionary conservation of the plant N-end rule pathway'. Plant Journal, 61 :741-751 [full-text] [Details]
    2010 'The plant N-end rule pathway: structure and functions'
    E. Graciet and F. Wellmer (2010) 'The plant N-end rule pathway: structure and functions'. Trends in Plant Science, 15 :447-453 [full-text] [Details]
    2009 'The N-end rule pathway controls multiple functions during Arabidopsis shoot and leaf development'
    E. Graciet*, F. Walter, D. OMaoilidigh, S. Pollmann, E. M. Meyerowitz, A. Varshavsky and F. Wellmer* (2009) 'The N-end rule pathway controls multiple functions during Arabidopsis shoot and leaf development'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 106 :13618-13623 [full-text] [Details]
    2006 'Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii. Functional consequences for glyceraldehyde-3-phosphate dehydrogenase'
    Lebreton S;Andreescu S;Graciet E;Gontero B; (2006) 'Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii. Functional consequences for glyceraldehyde-3-phosphate dehydrogenase'. FEBS Journal, 273 (14):3358-3369 [DOI] [full-text] [Details]
    2006 'Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen'
    E. Graciet, R-G. Hu, K. Piatkov, J.H. Rhee, E. M. Schwarz and A. Varshavsky (2006) 'Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 103 :3078-3083 [full-text] [Details]
    2005 'Rapid Communications in Mass Spectrometry'
    Delobel A.;Graciet E.;Andreescu S.;Gontero B.;Halgand F.;Laprévote O. (2005) 'Rapid Communications in Mass Spectrometry'. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 19 (22):3379-3388 [DOI] [full-text] [Details]
    2004 'Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex'
    E. Graciet, S. Lebreton, and B. Gontero (2004) 'Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex'. Journal of Experimental Botany, 55 :1245-1254 [full-text] [Details]
    2004 'Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation'
    E. Graciet, G. Mulliert, S. Lebreton, and B. Gontero (2004) 'Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation'. European Journal of Biochemistry, 271 :4737-4744 [full-text] [Details]
    2003 'The small protein CP12: A protein linker for supramolecular complex assembly'
    Graciet, E., Gans, P., Wedel, N., Lebreton, S., Camadro, J.-M., Gontero, B. (2003) 'The small protein CP12: A protein linker for supramolecular complex assembly'. Biochemistry, 42 (27):8163-8170 [DOI] [full-text] [Details]
    2003 'Modulation, via protein-protein interactions, of glyceraldehyde-3-phosphate dehydrogenase activity through redox phosphoribulokinase regulation'
    S. Lebreton, E. Graciet, and B. Gontero (2003) 'Modulation, via protein-protein interactions, of glyceraldehyde-3-phosphate dehydrogenase activity through redox phosphoribulokinase regulation'. Journal of Biological Chemistry, 278 :12078-12084 [full-text] [Details]
    2003 'Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for conformation changes upon association with the small protein CP12'
    E. Graciet, S. Lebreton, J-M. Camadro, and B. Gontero (2003) 'Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for conformation changes upon association with the small protein CP12'. European Journal of Biochemistry, 270 :129-136 [full-text] [Details]
    2000 'Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments'
    Wessel, P.M., Graciet, E., Douce, R., Dumas, R. (2000) 'Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments'. Biochemistry, 39 (49):15136-15143 [DOI] [full-text] [Details]

    Book Chapters

      Year Publication
    2017 'Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis'
    Thomson B;Graciet E;Wellmer F; (2017) 'Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis' In: Methods in molecular biology. USA: Clifton, N.J. [DOI] [Details]
    2016 'Molecular and regulatory mechanisms controlling floral organ development'
    Stewart D;Graciet E;Wellmer F; (2016) 'Molecular and regulatory mechanisms controlling floral organ development' In: [DOI] [Details]
    2014 'Next-generation sequencing applied to flower development: ChIP-Seq'
    Graciet E;O'Maoiléidigh DS;Wellmer F; (2014) 'Next-generation sequencing applied to flower development: ChIP-Seq' In: Methods in molecular biology. USA: Clifton N.J. [DOI] [full-text] [Details]
    2014 'Flower development: open questions and future directions'
    Wellmer F;Bowman JL;Davies B;Ferrándiz C;Fletcher JC;Franks RG;Graciet E;Gregis V;Ito T;Jack TP;Jiao Y;Kater MM;Ma H;Meyerowitz EM;Prunet N;Riechmann JL; (2014) 'Flower development: open questions and future directions' In: Methods in Molecular Biology - Flower Development. USA: Springer. [DOI] [Details]
    2014 'Genetic Control of Arabidopsis Flower Development'
    O'Maoileidigh, DS;Graciet, E;Wellmer, F (2014) 'Genetic Control of Arabidopsis Flower Development' In: MOLECULAR GENETICS OF FLORAL TRANSITION AND FLOWER DEVELOPMENT. LONDON: ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD. [DOI] [Details]

    Honours and Awards

      Year Title Awarding Body
    2007 EMBO Long Term Fellowship
    2004 HFSP Long-Term Postdoctoral Fellowship

    Professional Associations

      Association Function From / To
    Microbiology Society Member /
    Irish Plant Scientists Association Member /
    American Society of Plant Biologists Member /
    Society of Experimental Biology (SEB) Member /

    Committees

      Committee Function From / To
    Academic Council Member 2019 /
    Second year Biology Coordinator Coordinator 2020 /
    Departmental Teaching & Curriculum committee Member 2020 /
    Departmental Research Committee (Coordinator) Coordinator 2018 /
    Faculty Research Committee Committee member 2015 / 2019
    University Campus Life Committee Member 2019 /
    Biological Safety Committee Member 2016 /

    Employment

      Employer Position From / To
    Maynooth University Lecturer 02-JAN-14 /
    Trinity College Dublin/SFI SIRG Principal Investigator 01-OCT-09 / 31-DEC-13
    Trinity College Dublin Postdoctoral Research Fellow 01-APR-09 / 30-SEP-09
    EMBO fellowship EMBO postdoctoral fellow 01-APR-07 / 31-MAR-09
    California Insitute of Technology Postdoctoral research fellow (HFSP fellowship) 01-APR-04 / 31-MAR-07
    California Insitute of Technology Postdoctoral Research Fellow 01-AUG-03 / 31-MAR-04

    Education

      Year Institution Qualification Subject
    2003 Pierre and Marie Curie University (Paris 6) PhD
    2000 Institut National Agronomique Paris-Grignon MSc
    2000 Institut National Agronomique Paris-Grignon Diplome d'Agronomie Generale Agronomy

    Languages

      Language Reading Writing Speaking
    German Functional Functional Functional
    English Fluent Fluent Fluent
    Portuguese Functional Basic Functional
    Spanish Fluent Functional Fluent
    French Fluent Fluent Fluent

    Teaching Interests

    My primary teaching interests are plant biology and biochemistry.

    I lecture in the following modules:
    - BI201 - Biochemistry
    - BI305 - Literature review, including tutorials
    - BI306 - Plant Responses to the Environment
    - BI309 - General Methodology
    - BI440 - Control of Protein Activity and Abundance
    - BI425 - BioPharma - producing human therapeutics in plants

    I am also the second year coordinator for the Biology Department.


    Recent Postgraduates

      Graduation Student Name University Degree Thesis
    2019 Alexandra Miricescu Maynooth University PhD
    2019 Kevin Goslin Maynooth University PhD
    2014 Katarzyna Hanczaryk Trinity College Dublin PhD
    2010 Francesca Mesiti Trinity College Dublin MSc
    2009 Franziska Walter Trinity College DUblin MSc

    External Collaborators

      Name Role / Description Country
    Prof. Pitter Huesgen (Forschungszentrum Juelich) GERMANY
    Dr. Justin Lee (Leibniz Institute of Plant Biochemistry) GERMANY
    Prof. Markus Wirtz (University of Heidelberg) GERMANY
    Dr. Frederica Theodoulou (Rothamsted) UNITED KINGDOM
    Prof. Carl Ng (University College Dublin) IRELAND
    Dr. Ewen Mullins (Teagasc) IRELAND
    Dr. Susanne Barth (Teagasc) IRELAND